Dear all,
I heard comments lately, from two sources, that NextSeq 500 "requires extensive bioinformatics pipeline optimization and it has been very challenging for most labs with experience in NGS Illumina technology".
I personally believe they mean the bcl2fastq conversion, since both platforms use different chemistry. However, I want to know from you if any downstream analysis, following the generation of the fastq file, needs a special bioinformatics treatment or is challenging, as both sources claimed.
For me, I am not sure why treating a fastq file will differ either it is generated by NextSeq 500 or HiSeq or MiSeq !!!
Any comments/clarifications are much appreciated,
All best and thanks,
I heard comments lately, from two sources, that NextSeq 500 "requires extensive bioinformatics pipeline optimization and it has been very challenging for most labs with experience in NGS Illumina technology".
I personally believe they mean the bcl2fastq conversion, since both platforms use different chemistry. However, I want to know from you if any downstream analysis, following the generation of the fastq file, needs a special bioinformatics treatment or is challenging, as both sources claimed.
For me, I am not sure why treating a fastq file will differ either it is generated by NextSeq 500 or HiSeq or MiSeq !!!
Any comments/clarifications are much appreciated,
All best and thanks,
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