Gentra Puregene V.S. Genomic-tip (Qiagen)

[shiva]

Hi,

our lab is going to resequence the whole genome of a Drosophila melanogaster mutant strain using HiSeq. I found that Qiagen's Gentra Puregene and Genomic-tip are both used in literature for the genomic DNA preparation. Dose anyone have the experiences about which one gives better quality of the important starting materials for the downstream library preparation, sequencing... etc? Apart from the two kits, any protocols/kits that give good quality of Drosophila gDNA for next generation sequencing are all very appreciated.

Best regards,
Sue

[cement_head]

Hello,

I had the same question. I have been using Genomic-Tips (100) on the assumption that they gave excellent results (quality & yield), but I'm questioning the yields stated in the Qiagen manual. When I measure my yields by Qubit4 HS DNA assay (diluting sample), I find that I am achieving about 1/10th the yield stated in the GT manual(s).

Has anyone else experienced this same issue? Is it possible that the yields in the manual were reported using UV nanodrop, and thus might be inflated relative to a fluor-based assay?

----------------------------------------------------------------------------------------------------

EDIT: Most likely I've not resuspended the gDNA properly...