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1.8x volume should be good enough to get rid of <100bp fragments. 1x volume is good for <200bp. I have heard of different lots of SPRI beads giving different break points so YMMV. It shouldn't take any time to try out different volumes on some 100bp ladder, which is what we do.Leave a comment:
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SPRI bead for PCR cleanup in illumina pair end library preparation
Hi everybody,
I am preparing 300bp and 600bp illumina pair end library. Can anybody please let me know which SPRI beads and in what concentration should I use for cleaning up the PCR product in the end step. Thanks for the help.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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