Cluster metrics for individual barcodes

[garwuf]

Hi all,

I wonder if it is possible to assess some useful cluster metrics (FWHM or %>=Q30) not for the entire loaded pool but for individual barcodes (or maybe even for separate reads)? The Illumina SAV can show such stats for particular lanes/tiles but does not track the barcodes. In principle, each read can be tracked back to its location in the flow cell by coordinates extraced from the read name, but is there a way to relate it to a particular cluster and get its size?

[dpryan]

In the "stats/" directory produced by bcl2fastq you can find an XML file with per-tile metrics on %>=Q30 separated by barcodes.

[garwuf]

In the "stats/" directory produced by bcl2fastq you can find an XML file with per-tile metrics on %>=Q30 separated by barcodes.

Thanks a lot, I completely forgot about it.