Phix174 solexa reads repository

[mingkunli]

I need phix174 solexa data to do some test (the control DNA provided by ILLumina), any one know where I can find some?

Thanks.

[olesk]

I have put 25K phiX174 reads as test data to R2R here if thats what you - still - need:

http://milne.ruc.dk/R2R/

Look for "s_5_sequence" reads in "fastq, base 64" format

[mingkunli]

I have put 25K phiX174 reads as test data to R2R here if thats what you - still - need:

http://milne.ruc.dk/R2R/

Look for "s_5_sequence" reads in "fastq, base 64" format

Thanks for your reply, I still need them, but why these reads have such high quality score ("h" for most of the time)?

[olesk]

phiX174 data quality

Data were extracted as 25K consequtive reads extracted "not from the beginning" of the original file to avoid edge effects. I guess that high quality comes from 1) That Illumina overestimate the quality of their reads and 2) that this was a good day, where much longer reads could have been made, but this was not needed. You can have the full dataset if needed by sending me your mail as a private message.