Tweet
I would like to sequence genomes from 2 different organisms using Illumina Miseq. one is Staph. aureus of about 2.8Mbp and the other is a virus of about 13Kb. Is it okay to pool this samples after library preparation and run them in one flow cell? What are the pros and cons?
-
-
Sure. You will need to index (tag) the samples so the reads can be separate after the run. -
Yes, I will index the samples, but I was wondering if there is any effect of having different sizes of genomes pooled together. Will the larger be 'sequenced more' than the smaller or will they get equal representation in the final output? Is there a cut-off of or guideline of what kinds of genomes you can pool together?
I am new at this so it may seem like a silly question...Comment
-
The number of reads for each library is dependent on loading not the genome size. Pooling libraries with same fragment length distribution should output reads in proportion to loading. If the library (not genome) sizes are different, then with equal loading more of shorter libraries will be sequenced.
You can pool many types of libraries in one run if the sequencing configuration is similar.Comment
-
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...06-06-2024, 07:15 AM -
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...05-24-2024, 01:16 PM
Comment