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  • deepakpatilp
    Junior Member
    • Jun 2010
    • 8
    #1

    Sequencing for PCR products of different sizes

    Hi! Friends

    We have constructed directional libraries for RNAseq and we are interested in sequencing a range of PCR products (150-1000 bp) for sequencing. Can anyone tell me how much sample should be submitted for illumina sequencing without affecting cluster density? If concentration is in molar terms, how should I calculate this?

    Regards
    Deepak
    DPP
    Tags: None
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