12 mer for Miseq custom sequencing primer
Hi~
Does anyone had read the article below?
Treseder et al., 2016
http://onlinelibrary.wiley.com/doi/1...C13B9AE.f03t04
According to the Material and Methods (attachment), they wanted to use stagger strategy from (Tremblay et al., 2015) but the PAD region has only 12bp, I used custom Index primer and custom R2 sequencing primer based on PAD sequence for a Miseq sequencing run but got a fail run when index run had started. I think the 12mer Index primer might be the problem. Here's me custom primer
Index sequencing primer
GGCTGACTGACT
Read 2 sequencing primer
AGTCAGTCAGCC
Can anyone help me for this issue?
Does anyone had read the article below?
Treseder et al., 2016
http://onlinelibrary.wiley.com/doi/1...C13B9AE.f03t04
According to the Material and Methods (attachment), they wanted to use stagger strategy from (Tremblay et al., 2015) but the PAD region has only 12bp, I used custom Index primer and custom R2 sequencing primer based on PAD sequence for a Miseq sequencing run but got a fail run when index run had started. I think the 12mer Index primer might be the problem. Here's me custom primer
Index sequencing primer
GGCTGACTGACT
Read 2 sequencing primer
AGTCAGTCAGCC
Can anyone help me for this issue?