Hi guys Im new to NGS and have illumina runs from a pooled data set of 8 organisms. There are ~17 million sequences of 85bp long that I am using for de novo assembly. I would like to separate the reads into files based on the organism they belong to. To do this I need to find some unique identifier in each read header, eg:
HWI-EASXXX_0012:7:1:0:892#ACAGTG/1:
The problem is instead of an index number I have multiplexer tags of the form NNNNNN at the end of the header. With so many different tags there doesnt seem to be any way to separate organisms based on these tags. Has anyone come across this problem before or have any suggestions to alternate routes? This is my first illumina data set so if Im missing out on some obvious answer, I apologize in advance.
HWI-EASXXX_0012:7:1:0:892#ACAGTG/1:
The problem is instead of an index number I have multiplexer tags of the form NNNNNN at the end of the header. With so many different tags there doesnt seem to be any way to separate organisms based on these tags. Has anyone come across this problem before or have any suggestions to alternate routes? This is my first illumina data set so if Im missing out on some obvious answer, I apologize in advance.