Miseq- error

[María Camila Fetiva]

Hello,
My miseq showed an error and stoped running two hours after the run started.
Error: Through- focus peak did not exceed SNR threshold (Illumina.Instrument.Bolt.Logic.AutoFocus.DarkSampleException)</ErrorDescription>

It was using a V2X 500cycles kit with 24 samples from SOPHIA Genetics NGS Panel.

Could you explain me which could be the reason and what could be the solution?

[Jessica_L]

Hi Maria,

I'm not familiar with this exact error message but it sounds like the machine may have had difficulty finding a plane of focus for the flow cell.

The very first thing you should do is contact Illumina tech support and they can help you troubleshoot your instrument and failed run. If you need additional help troubleshooting libraries, you can reply to this thread and I'll see if I can help you.

Good luck!