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  • junoguti
    Junior Member
    • Sep 2017
    • 1
    #1

    Scale

    Hi! My lab wants to do a pilot about skin microbiome (which I am not an expert) and I need to request a quote for V1-V3 primers, the company asked me which scale for synthesis I want the oligos.
    According to some websites 25nmil seems reasonable.
    Any thoughts to help me?

    thank you
    Last edited by junoguti; 09-14-2017, 02:36 PM.
    Tags: None
    • nucacidhunter
      Jafar Jabbari
      • Jan 2013
      • 1250
      #2
      25 nmole with standard desalting is OK and should last 100s of reactions. Depending on oligo length or modifications, oligos might need PAGE or HPLC purification which would require higher synthesis concentrations and this differ among manufacturers.

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