Hi everyone,
we have troubles with our MiSeq Runs. At about cycle 80 we have a massive decrease in Q30scores.In general the quality of the runs is not good. Q30 scores of 50 or lower. In addition, during my last two runs- the intensity does not increase . I am very new to this technique and have therefore attached some Plots from basespace.
Maybe someone had the same problem? Or has an idea about what is going wrong- what are the possible causes? I went through the troubleshooting guides, but could find yet any answer.
thanks :-)
we have troubles with our MiSeq Runs. At about cycle 80 we have a massive decrease in Q30scores.In general the quality of the runs is not good. Q30 scores of 50 or lower. In addition, during my last two runs- the intensity does not increase . I am very new to this technique and have therefore attached some Plots from basespace.
Maybe someone had the same problem? Or has an idea about what is going wrong- what are the possible causes? I went through the troubleshooting guides, but could find yet any answer.
thanks :-)
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