Hello,
Does anyone have any experience sequencing samples on the Illumina platform that have been whole-genome amplified? Has anyone explicitly tested whether it introduces allelic/amplification bias that could be a problem for SNP calling?
We've heard rumors and impressions that say it's a problem and should be avoided. But we have valuable samples that we would like to sequence and WGA seems to be the only way.
Thanks in advance.
Does anyone have any experience sequencing samples on the Illumina platform that have been whole-genome amplified? Has anyone explicitly tested whether it introduces allelic/amplification bias that could be a problem for SNP calling?
We've heard rumors and impressions that say it's a problem and should be avoided. But we have valuable samples that we would like to sequence and WGA seems to be the only way.
Thanks in advance.
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