CBot vs. Cluster station

[cnicolet]

Hello:
Illumina says the cBot will give slightly higher cluster numbers than the cluster station with the same library concentration. Has anyone seen this? How much higher? We are using 10 pM for the library currently, to get 350,000/mm2 on the old cluster station.

Thanks for your help!

CN

[lwwhu]

I guess it depends on your library quantification, too. We constantly get 400K/mm2 with 6.5 pM input (on cBot).

[NextGenSeq]

The cBot is definitely more consistent and gives higher cluster numbers. We get more like 500 to 600K/mm2 PF clusters

[mnkyboy]

I guess it depends on your library quantification, too. We constantly get 400K/mm2 with 6.5 pM input (on cBot).

I get about that for passing filter clusters with RNA-seq libraries on the cBOT.