Hi everyone,
Yesterday i did a 16s MiSeq run using custom primers, when i tried to demultiplex using samplesheet generated through IEM, i found error during secondary analysis to generate Fastq files. I replaced the illumina index sequences with custom index sequences during samplesheet generation. But still I could not overcome the issue. It would be great help, if any one can help to sort this issue. Many thanks in advance.
Yesterday i did a 16s MiSeq run using custom primers, when i tried to demultiplex using samplesheet generated through IEM, i found error during secondary analysis to generate Fastq files. I replaced the illumina index sequences with custom index sequences during samplesheet generation. But still I could not overcome the issue. It would be great help, if any one can help to sort this issue. Many thanks in advance.
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