Does anyone know how many cycles one can realistically push from a 600-cycle kit (does the SBS software even allow you to specify this any more?), and what happens when you run out of reagent? I am assuming the run, sans final cycle, is still good...
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625 according to Illumina https://support.illumina.com/bulleti...in-my-kit.html. Be avare that if you use dual indexing, there will be seven "dark" cycles in addition to what you specify in the sample sheet.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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