Hello everyone,
As the title, I ran a sequencing reaction last week on MiniSeq system by Mid-Output kit 300 cycles. I determined the concentrations of library by BioAnalyzer HS chip and diluted the pooled library to 1.2 pM. When the reaction completed, the system notified me that it was undercluster (only 141k/mm2). Two points make me confused that: (1) I have optimized the concentration of library to 1.2 pM already and it was good for other runs;(2) Illumina said that the library should be diluted to 1.1 pM if I use BioAnlyzer as a quantification methods.
I hope that someone met the similar problem with me and give me some suggestions.
As the title, I ran a sequencing reaction last week on MiniSeq system by Mid-Output kit 300 cycles. I determined the concentrations of library by BioAnalyzer HS chip and diluted the pooled library to 1.2 pM. When the reaction completed, the system notified me that it was undercluster (only 141k/mm2). Two points make me confused that: (1) I have optimized the concentration of library to 1.2 pM already and it was good for other runs;(2) Illumina said that the library should be diluted to 1.1 pM if I use BioAnlyzer as a quantification methods.
I hope that someone met the similar problem with me and give me some suggestions.