Coverage

[suludana]

Hi,
I need an opinion.
If I want to read 5 Mb in a line of a GAI flow cell I will obtain an approximate 30x coverage in a single run. In this case the paired end is not necessary, because of the high coverage. I`m I right?
Thanks.

[swbarnes2]

I've looked at a lot of bacterial samples that have genomes that size, and a single lane of single-end sequencing will give you about that coverage.

Paired ends sequence will help you figure out everything is put together, but if you are confident that your reference is right (like it's a region of human or mouse or some other well-known organism), then maybe you don't need that.