Hi,
I am writing my thesis and I ran into a question. Maybe someone here happens to know the answer to this.
We have an Illumina Miseq in our lab. For our sequencing approach we used custom sequencing primer (well 18 and 20 on the MiSeq cartridge). I know that these primers are used during the sequencing process itself. But are they also used for the bridge amplification that precedes the actual sequencing? Or does the polymerase for the bridge amplification start directly at the Illumina adapter and the flow cell.
I hope I phrased this clearly enough. Any insight would be much appreciated.
Thanks in advance.
I am writing my thesis and I ran into a question. Maybe someone here happens to know the answer to this.
We have an Illumina Miseq in our lab. For our sequencing approach we used custom sequencing primer (well 18 and 20 on the MiSeq cartridge). I know that these primers are used during the sequencing process itself. But are they also used for the bridge amplification that precedes the actual sequencing? Or does the polymerase for the bridge amplification start directly at the Illumina adapter and the flow cell.
I hope I phrased this clearly enough. Any insight would be much appreciated.
Thanks in advance.
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