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  • BAD HiSeq flowcells

    What percent of your HiSeq flowcells have been 'bad'; ie have the wavy lines of clusters? Recently we have been experiencing >50% of our flowcells with this problem and are quite frustrated and disappointed in Illumina's QC. Just curious what other labs are experiencing.

    Thanks

  • #2
    Similar for us. About half have been wavy phenotype and the remainder have a black stripe running down the middle of the tile. Illumina has told us that the stripe represents less than the 10% variation that they consider standard for inter-flowcell differences and so don't worry about it unless, as with the wavy phenotype it starts drastically affecting data output.

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    • #3
      almost 100%

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      • #4
        approximately 40%

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