BAD HiSeq flowcells

[BIG_SNP]

What percent of your HiSeq flowcells have been 'bad'; ie have the wavy lines of clusters? Recently we have been experiencing >50% of our flowcells with this problem and are quite frustrated and disappointed in Illumina's QC. Just curious what other labs are experiencing.

Thanks

[Jessica_L]

Similar for us. About half have been wavy phenotype and the remainder have a black stripe running down the middle of the tile. Illumina has told us that the stripe represents less than the 10% variation that they consider standard for inter-flowcell differences and so don't worry about it unless, as with the wavy phenotype it starts drastically affecting data output.

[cella]

almost 100%

[nextgen2010]

approximately 40%