Hi all,
We are designing an experiment in which we are interested in measuring 100+ transcriptomes using Illumina mRNA sequencing. I wonder whether anyone who is currently doing multiplexing for transcriptome analysis has any experience to share about relative depth of coverage at different levels of multiplexing. What would be the maximum number of transcriptomic samples you would be comfortable combining in a single lane with the GAII? (We work with yeast.)
Many thanks,
Andrea
We are designing an experiment in which we are interested in measuring 100+ transcriptomes using Illumina mRNA sequencing. I wonder whether anyone who is currently doing multiplexing for transcriptome analysis has any experience to share about relative depth of coverage at different levels of multiplexing. What would be the maximum number of transcriptomic samples you would be comfortable combining in a single lane with the GAII? (We work with yeast.)
Many thanks,
Andrea
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