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  • CatWoman
    Junior Member
    • Feb 2019
    • 2

    #1

    concentrating Nextera XT libraries

    Hi, We need to concentrate Nextera XT DNA libraries before baiting with MyBaits. The libraries are 22ul and total DNA ranges from 130-160ng. The minimum recommended input DNA for baiting is 100ng and volume is 7ul. Do others have experience of best way to concentrate Speedvac? MinElute? Or something else. These are clinical samples and there is a very limited amount of DNA left.
    Extraordinarily grateful for any/all advice offered
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250

    #2
    If libraries are eluted in water, SpeedyVac is the best option otherwise Zymo DNA Clean & Concentrator (DCC-5) which allows elution with minimum 6 ul.

    Comment

    • CatWoman
      Junior Member
      • Feb 2019
      • 2

      #3
      concentrating Nextera XT libraries

      Thank you very much for your help

      Comment

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