Originally posted by james hadfield
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In my post above I was not clear about what I thought MiSeq might be in comparison to GA or HiSeq capacity. I meant 10% of a lane "The imaging for ten ‘tiles’ would be about 30sec.".
If ten tiles is correct then doubling this would only add about two hours to a run to double data volume. Maybe Illumina will make this bit flexible as well. Then users could dial in run time and data volume.
BTW, many people have already done this on GA or HiSeq.
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A little more info from the UK rep here
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I was wondering if it might be possible to get even more fomr this box of tricks by daisychaining some of the internals.
Right now the imaging module is the most expensive bit in a GA or HiSeq. This is probably the same for MiSeq. As imaging takes 30sec compared to five mnutes for chemistry why not simply have six fluidics modules and put six 10 tile single lane flowcells on, or just one. All would be imaged by the same module just like HiSeq.
MiSeq 2000. You heard it here first ;-)
James.
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Thanks, Nick. I see now that that was on your first blog post. Is that price inclusive of library prep?
If so, MiSeq is winning on every metric -- not least that it will use the exact same libraries as the big machine. As someone else said, it's not really in our interest for Illumina to leave its competition in the dust, but that's what seems likely here.
Assuming the machine meets these specs. I guess we do know by now that that's a big assumption.
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It sounds like the same reagents on a lower capacity machine. I think many labs would rather not go to the trouble, especially when you consider that the amount of labor is the same. Also, any word about machine cost? I get the feeling they are trying to compete with IonTorrent.
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Originally posted by GERALD View PostIt sounds like the same reagents on a lower capacity machine. I think many labs would rather not go to the trouble, especially when you consider that the amount of labor is the same. Also, any word about machine cost? I get the feeling they are trying to compete with IonTorrent.
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