Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Gurs
    Junior Member
    • Mar 2019
    • 2

    #1

    running RTA offline

    Hi,
    After some data of HiSeq2500 run were accidentally deleted, I have the following files for each tile in the 'Processed' folder:
    *.errorMap
    *.qms
    *.stats
    *.FWHMMap
    *.align
    *.bqm
    *.readErrors
    *.bqm
    *.clocs
    *.control
    *.ctr
    *.filter
    *.tempdensity
    But I don't have *.bcl or *.cif files.
    Is there any option to create bcl files for this run? My RTA version is 1.18.66.4. I saw that there is an option to run RTA offline (OLB, off-line base-calling), but when I run it (double-click the RTAstart.bat file) I get the message that bcl files are already generated.
    Thanks for helping.
  • misterc
    Member
    • Jan 2016
    • 46

    #2
    No, without .bcl or .cif files you're stuck.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      Yesterday, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    71 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...