Hello and dark digit cycle on HiSeq and NovaSeq

[NGS_Man]

Hi at all,

im new in the forum and i want say just hi. Hope we have a nice time.

Anyway, my question is about the dark digit cycles on HiSeq and NovaSeq.

First, what does it mean and what is happening in this cycles??
How many cycles are dark digits and so on...


Thanks and best regards,

NGS_Man

[GenoMax]

Look on page 5 of this document from Illumina. I believe you are referring to "chemistry-only" cycles described there.

There is also a past thread on this: http://seqanswers.com/forums/showthread.php?t=29852

[NGS_Man]

Thanks a lot, genomax