Hi,
I have some DNA samples of water and ice and I want to prepare 16s library.
3 samples out of 13 don't have any bands after amplicon PCR. Their DNA concentrations are 19.1, 14.7, and 15.2 ng/ml measured by Qubit. I was able to see bands for other samples with lower DNA concentrations.
Does anybody have any idea?
Thanks
I have some DNA samples of water and ice and I want to prepare 16s library.
3 samples out of 13 don't have any bands after amplicon PCR. Their DNA concentrations are 19.1, 14.7, and 15.2 ng/ml measured by Qubit. I was able to see bands for other samples with lower DNA concentrations.
Does anybody have any idea?
Thanks
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