Is it possible to increase the number of samples of NextSeq 500 v2.5 high output flow cell beyond the recommended amount of 12 samples for exome sequencing? Has it been done before?
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As long as you have a unique set of index sequences it is certainly possible. The total yield from a FC is not going to change so that final read number will get divided into the samples you put in the pool i.e. each sample will get proportionately less number of reads. -
Thank you for your reply GenoMaxComment
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