Kapa lib quant question

[wei210]

Hello. I am new here. I have a naive question. When we make series dilution for kapa lib quant.
1) Will it make big difference if dilute the lib using H2O vs 10mM tris PH8.0 w 0.05% tween-20 ?
2) will kapa conc typically higher than the Qubit conc (lib w Illumina p5/p7 sequence primer) ?
3) vetex to mix should be better than Pipet up/down. In the case using Pipet up/down. How many times up/down should be sufficient?
Thank you all so much

[seq198]

For PCR-free libs kapa quant kit makes sense, as qubit will not deliver accurate results. he different buffers for diluting should not have a big effect for what I can say. 5 times pipetting up and down should be sufficient.

[wei210]

Thank you so much, Seq198