I used Lexogen's 3' Quantseq kit and mistakenly used the wrong bead amount for the first cDNA purification (30 ul beads instead of 16). My guess is that resulted in libraries with lower fragment size distribution than I normally see, and several libraries have obvious peaks around 150 bp indicating likely adapter dimer (see attached trace). I was hoping to sequence these libraries with 75 bp SR on Next-seq. What should I expect to get out of the sequencing run if I proceed? I am contemplating trying Ampure Xp purification, but the libraries are already small (most around 300 bp average, or less). So I don't know how it would work. I have some reads to burn so it would be ok if I lost some to adapter dimer (maybe even as much as 30% or 40%). But if most the reads would be lost, I would probably just remake the libraries.
-
-
Dear QuantSeq kit user, please send these details to [email protected], and our technical support will reply ASAP.
Thank you,
Lukas Paul
Sr. Manager Scientific Affairs
Lexogen GmbH
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment