Tweet
I used Lexogen's 3' Quantseq kit and mistakenly used the wrong bead amount for the first cDNA purification (30 ul beads instead of 16). My guess is that resulted in libraries with lower fragment size distribution than I normally see, and several libraries have obvious peaks around 150 bp indicating likely adapter dimer (see attached trace). I was hoping to sequence these libraries with 75 bp SR on Next-seq. What should I expect to get out of the sequencing run if I proceed? I am contemplating trying Ampure Xp purification, but the libraries are already small (most around 300 bp average, or less). So I don't know how it would work. I have some reads to burn so it would be ok if I lost some to adapter dimer (maybe even as much as 30% or 40%). But if most the reads would be lost, I would probably just remake the libraries.
-
-
Dear QuantSeq kit user, please send these details to [email protected], and our technical support will reply ASAP.
Thank you,
Lukas Paul
Sr. Manager Scientific Affairs
Lexogen GmbH
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment