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  • kluttgeharm
    Junior Member
    • Jun 2019
    • 1
    #1

    Sequencing through the insert

    Hi I am wondering how common it is for libraries to sequence through the adapter and run out of nucleotide to sequence like in this Illumina video. (https://www.youtube.com/watch?v=bkMiy5lGIqs)

    Is it recommended to analyze the size of you library prior to deciding on read lengths?
    Last edited by kluttgeharm; 07-24-2020, 06:06 AM.
    Tags: None
    • luc
      Senior Member
      • Dec 2010
      • 469
      #2
      Yes, you definitely want to QC your library for the fragment lengths before sequencing using a Bioanalyzer or equivalent devices.

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