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  • NewbtoNGS
    Junior Member
    • Aug 2020
    • 4

    #1

    Will my run fail?

    I recently loaded a MiSeq that had low library yields ~1-2nM. Does this indicate my sequencing run will fail? Even though the Q30 and PF look good?

    Will I get usable data from this?
  • luc
    Senior Member
    • Dec 2010
    • 469

    #2
    The run is still ongoing?
    More information would be rquired.

    Comment

    • NewbtoNGS
      Junior Member
      • Aug 2020
      • 4

      #3
      Yes. The run is still running. %PF 91%, Q30 92%.

      Comment

      • luc
        Senior Member
        • Dec 2010
        • 469

        #4
        I beleive there is no reason to expect the run to fail now. The data should be usable as indicated by the qualityscores.

        Comment

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