DGE on procaryotic organisms

[huguesparri]

Hello,

We would like to perform DGE on a procayotic organism (a symbiotic bacteria).
Unfortunately, Illumina's DGE sample prep. kit is based on mRNA selection using oligo(dt) magnetic beads.

Considering that we find a way to enrich our sample in mRNA (it seems that there are some kits that can get rid of some of the ribosomal RNA), does anybody have experience in procaryotic mRNA bank construction?
Is it possible?
Any help would be welcome.
Thanks in advance

H.

[palmer55]

Why DGE?

Hi huguesparri,

Any reason to use DGE while mRNA Seq should be more applicable?

Yoder-Himes DR, Chain PS, Zhu Y, et al. (2009) Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing. Proc Natl Acad Sci U S A 106: 3976-81