Hello, I am new on the website and i would like to know if someone could help me with the interpretation of some results. I did not find enough information on the following inks:
I am looking for insertion deletion. Here are the example of 2 samples.
sample1 56 * */+CTT 87 87 37 9 * =+CTT 7 2 0 0 0
sample2 56 * =+CTT/+CTT 25 1291 37 21 =+CTT * 18 3 0 0 0
For sample 1, I understand that there are 7 reads on 9 that agree with the insertion of CTT at position 56 while 3 did not.
For samples 2 I understand that there are 18 reads on 21 that agree with the insertion of CTT at position 56 while 3 did not.
I do not inderstand what is the difference between "*/+CTT" and "=+CTT/+CTT"
Moreover i do not understand why in sample 1, in the column 9 there is a "*" and then in column 10 it is written "=+CTT", while for sample 2 , in column 9 it is written "=+CTT" and then in column 10 "*". What is the difference?
If you know also a website were i could see examples, that would be also great, Thank you
I would really appreciate if someone could help me, thank you so much
Best regards
Labmtl
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM