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  • Vita9285
    Junior Member
    • Jan 2022
    • 3

    #1

    Run status "Uploading" lasts for 10 hours (NextSeq500)

    Hello everyone! I need help.
    Our last run is still on uploading stage.
    Is any way to trigger the next step (fastq generation)?

    Instr: NextSeq500
    Illumina Basespce

    Thank you
  • IonVsIllumina
    Junior Member
    • Oct 2013
    • 2

    #2
    This happened to me a few times. It was due to the internet connection dropping while the run was going/uploading. I contacted illumina basespace tech support. I verified with them that the entire run was uploaded and they manually triggered the fastq gen script on their end.

    Comment

    • Vita9285
      Junior Member
      • Jan 2022
      • 3

      #3
      Thank you. We contacted Illumina for support. In our case, the solution was to turn the instrument off and on again (facepalm)

      Comment

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