Hi,
I am new to sequencing stuff by myself and till date I have ran at least 30 miniseq runs. Some runs were decent, but most of the runs over clustered in spite of quantifying libraries using NEB library quant kit. I make my own libraries using two sets of PCR and custom designed barcodes, purify and run bioanalyzer. Is there a way to troubleshoot what is exactly wrong? I did contact Illumina and they haven't given me a satisfactory answer ever. Any help would be appreciated.
I am new to sequencing stuff by myself and till date I have ran at least 30 miniseq runs. Some runs were decent, but most of the runs over clustered in spite of quantifying libraries using NEB library quant kit. I make my own libraries using two sets of PCR and custom designed barcodes, purify and run bioanalyzer. Is there a way to troubleshoot what is exactly wrong? I did contact Illumina and they haven't given me a satisfactory answer ever. Any help would be appreciated.
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