small RNA-seq library prep
Hi
I have total RNA from human cancer samples, the total RNA to start with had RIN ranging from 7 to 5.5. accordingly when I have made the small RNA seq library according to the bioanalyzer reports only 50-33% of the products belong to the expected small RNA. Is it worth sequencing these library and can I get good enough data for comparing the three samples with 50%, 38% and 33% of small RNA products?
Please advice!
I have total RNA from human cancer samples, the total RNA to start with had RIN ranging from 7 to 5.5. accordingly when I have made the small RNA seq library according to the bioanalyzer reports only 50-33% of the products belong to the expected small RNA. Is it worth sequencing these library and can I get good enough data for comparing the three samples with 50%, 38% and 33% of small RNA products?
Please advice!