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  • #16
    The edge of some flowcells didn't have the oligos deposited correctly for the bridge amplification. This was very evident by looking at the thumbnail images.

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    • #17
      Originally posted by NextGenSeq View Post
      For poor libraries we'll see 80 million reads per lane or less if they are really bad. Good libraries give 100 to 130 million per lane.

      Once we began quantifying our libraries by QPCR we get much more consistent cluster densities. We get good results using the NuGen kits for PE genomic libraries and the protocol is very fast and easy.
      What kind of cluster densities do you see with 100-130million reads? How many pM are you loading to get that number of reads?

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      • #18
        Sorry for this naive question but, what is the % Phas./Preph.?

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