The edge of some flowcells didn't have the oligos deposited correctly for the bridge amplification. This was very evident by looking at the thumbnail images.
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What kind of cluster densities do you see with 100-130million reads? How many pM are you loading to get that number of reads?Originally posted by NextGenSeq View PostFor poor libraries we'll see 80 million reads per lane or less if they are really bad. Good libraries give 100 to 130 million per lane.
Once we began quantifying our libraries by QPCR we get much more consistent cluster densities. We get good results using the NuGen kits for PE genomic libraries and the protocol is very fast and easy.
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
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by SEQadmin2
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...-
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