[link1]

small mirna protocol

westlook, not sure what that sequence is....screened my results, haven't seen it.

for the other folks in the forum, I've gotten the miRNA protocol to work consistently now (30th library today). the most important things I've found are the quality of your starting material and that the ratio of miRNA to adenylated adapter should be ~ 1:10. Let me know if you're having trouble and I can help.

[bomper]

Hi,

I'm using the Illumina small RNA v1.5 kit and i would like know what is the yield. Anybody knows it?
Many Thanks!

[DMO]

Has anyone attempted prepping small RNA from degraded Total RNA (RIN 2.5-3)? Any luck?

[cascoamarillo]

Hi,

I do have the same question; someone attempting to do a small RNA library from total RNA vs. previously selected small RNA. Does it carry any difference in the final gel (clear bands, lost of potential material...)?. Has anyone done this with Illumina, NEB, etc. small RNA kits?

Regards.

[nirwalniraj]

I had prepared library for c. elegans small RNA sequencing using v1.5 small RNA illumina kit. I used 6% native DNA-PAGE to run the samples and cut down only the band near to 100 bp. I should got 93 and 100 bp bands in all the samples after PCR enrichment but i got only 100-102 bp and 106-109 in the other samples after agilent bioanalyzer analysis. Please suggest me, whether i should go for downstream processing (sequencing by genome analyzer) or not with these samples.