Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • howiewang
    Junior Member
    • Apr 2011
    • 1

    #1

    Illumina primer immobilization

    I'm just curious the primers are actually immobilized on the bottom wall only or they are immobilized on both top and bottom wall (and side wall as well) of the flow cell?
    If primers are on bottom wall only, how the bottom wall is selectively activated?
    If primers on both bottom and top wall, if the fluorescence signal from top wall will effect the final signal (fluorescence from out of focus plane)? or illumina actually image both walls?
    Many thanks.
    Last edited by howiewang; 06-01-2011, 05:45 PM.
  • james hadfield
    Moderator
    Cambridge, UK
    Community Forum
    • Feb 2008
    • 224

    #2
    Oligos are attached al over the surfaces of the lane. The imaging is directed to one focus point to generate high quality data.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-13-2026, 09:56 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-09-2026, 11:14 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Working...