So I can't find the formulation/concentration of the Low TE that comes with the Ion kits anyone know what the concentration and pH of the Tris-EDTA should be?
-
-
Low TE generally is 10 mM Tris pH 8 0.1 mM EDTA, don't know for sure if Ion Torrent uses that. -
-
Hi there
Is this Low TE then acceptable to use for diluting gDNA in the Ion Shear fragment Xpress kit? I did an ethanol precipitation and re suspended the gDNA in RNase-free dH2O to be safe but I would prefer to use it as is, if it wont affect the reaction.
JuliJuli Hepple (MSc Genetics)Central analytical facilitiesDNA Sequencing UnitSouth AfricaComment
-
Yes, I would use this buffer to resuspend your DNAs.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment