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Alternative (cheaper) reagents for Ion Torrent PGM sequencer

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  • Alternative (cheaper) reagents for Ion Torrent PGM sequencer

    Hi everyone,
    in my lab we are trying to find a way for reduce the running costs of our new IonTorrent PGM sequencer... any suggestion?

    Anyone found cheaper reagents that can substitute the ones sold by Life Technology?

  • #2
    We have checked out NEB kits, work OK (although still more expensive than I'd like).

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    • #3
      Originally posted by genseq
      Cheap barcoding:
      Looks really interesting, time to use google translator
      Last edited by Gorbenzer; 06-05-2012, 11:29 PM.

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      • #4
        Does anybody know the sequences of primers, adaptors and immobilized oligos?

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        • #5
          genseq - thanks for the latest info!

          How about the NEXTflex kits? Anyone had experience with their DNA-Seq kits or the barcoding?
          http://www.biooscientific.com/Detail...A%20Sequencing

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          • #6
            Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...

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            • #7
              Originally posted by prooney View Post
              Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...
              This is intresting question. Nobody ever tried?

              I did it with the cancer hotspot panel. Sequencing results are similar.
              Last edited by salyamoff; 04-03-2014, 11:23 AM.

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