I need Illumina and Ion Torrent datasets for same organism and strain for my project work. Reference genome should also be available. I searched on Ion community, NCBI, DRA search but could find only 2-3 datasets which can be used in my project. I cannot use human genome datasets as it is too large. Can someone suggest me some other websites where I can find some more real datasets. Thanks.
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Look in http://www.ncbi.nlm.nih.gov/pubmed/22827831 & http://www.ncbi.nlm.nih.gov/pubmed/22522955. You should be able to find a number of E.coli K12 MG1655 datasets; that is the sample that the platforms love to use for test runs.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...-
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
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