Tweet
Cancer panel v2,low quality,316v2
Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?
-
-
We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard
JPC -
Thank you for your reply!Originally posted by JPC View Post
We had try that before, but it didn't work!
by the way, how do you quantify the library, 2100 or qubit?Comment
-
qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)
JPCComment
-
-
You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out.Comment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment