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  • Cancer panel v2,low quality,316v2

    Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?

  • #2
    We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard

    JPC

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    • #3
      Originally posted by JPC View Post
      We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard

      JPC
      Thank you for your reply!
      We had try that before, but it didn't work!
      by the way, how do you quantify the library, 2100 or qubit?

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      • #4
        qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)

        JPC

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        • #5
          Originally posted by JPC View Post
          qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)

          JPC
          thank you very much

          Comment


          • #6
            You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out.

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