Any ideas as to how to increase target coverage. Our current panel has over 50 regions that require sanger to back-fill. The vast majority of these regions are due too low or poor coverage (less than 30 reads). We run an ampiseq 200bp on a 316 chip. We have tried a 318, and lowering the input gDNA. Thank you.
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have you tried resubmitting your ampliseq design with the latest version? -
sanger regions
Yes, and it made little difference. There was some slight improvement, but this is all in silico and we have found that to be an underestimate. Thanks.Comment
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are these in difficult regions? If not, maybe you can just ask them to add your sanger primers?Comment
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