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  • flameout
    Junior Member
    • Jul 2014
    • 1
    #1

    Transcriptome analysis using Ion PGM

    Has anyone got experience with transcriptome studying using Ion PGM? What reagent kits would you recommend for library preparation?

    Is the 318 chip sufficiently "capacious" to analyze the whole-genome transcription of a bacteria (for instance, E.coli)?

    Sorry for many questions at once. I'm new to NGS and would like to figure out some preliminary issues for my future experiments.
    Thanks for your opinion and advice!
    Tags: None
    • snetmcom
      Senior Member
      • Oct 2008
      • 159
      #2
      You can do whole genome sequencing of e.coli on a 316 chip, so you can easily fit several transcriptomes on a 318 chip.

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