Hi All
I am analysing the RNA-Seq from Ion Proton.
The output file of each running is shown as follows (figure 1)Untitled.png
I think the RNA_Barcode_None_001 is the sample file I need to do analysis, am I right?
Question 1: But what is the other fastq file (barcode name: no barcode)?
And for this figure (figure 2),
Question 2: is this output file contain two samples?
However, if the '' No barcode '' file is very big (figure 3) , The QC plot of 'No barcode' and 'RNA_Barcode_None_001' are separately shown in figure 4 and figure 5.
Question 3: is it normal? What happened? Could it be wrong label or a bad sample or an unseccessful sequencing?
Thank you!
I am analysing the RNA-Seq from Ion Proton.
The output file of each running is shown as follows (figure 1)Untitled.png
I think the RNA_Barcode_None_001 is the sample file I need to do analysis, am I right?
Question 1: But what is the other fastq file (barcode name: no barcode)?
And for this figure (figure 2),
Question 2: is this output file contain two samples?
However, if the '' No barcode '' file is very big (figure 3) , The QC plot of 'No barcode' and 'RNA_Barcode_None_001' are separately shown in figure 4 and figure 5.
Question 3: is it normal? What happened? Could it be wrong label or a bad sample or an unseccessful sequencing?
Thank you!
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