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  • feng
    Member
    • Oct 2010
    • 50

    #1

    low quality of control ISP

    Hi all, any suggestion on lower quality on test fragments? Thanks in advance.
    Attached Files
  • snetmcom
    Senior Member
    • Oct 2008
    • 159

    #2
    What's the 50aq17, and was there a data issue?
    Sometimes the TF's get old, but the run is completely fine.

    Comment

    • feng
      Member
      • Oct 2010
      • 50

      #3
      Thanks. Snetmcom. Any suggestions? Thanks a lot.

      Alignment Quality AQ17 AQ20 Perfect
      Total Number of Bases [bp] 5 G 3.9 G 3.3 G
      Mean Length [bp] 88 76 67
      Longest Alignment [bp] 281 272 258
      Mean Coverage Depth [x] 1.6 1.3 1.1

      Comment

      • snetmcom
        Senior Member
        • Oct 2008
        • 159

        #4
        The 50AQ17 metric of the TF's, not the run metrics.

        Comment

        • feng
          Member
          • Oct 2010
          • 50

          #5
          40% percent 50AQ17.

          Comment

          • feng
            Member
            • Oct 2010
            • 50

            #6
            The control ISPs were just from a new kit.

            Comment

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